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人白介素2受體酶免試劑盒,(IL-2R)ELISA檢測(cè)試劑盒 ,英文名: IL-2R ELISA Kit ,英文縮寫: IL-2R ,常見試劑盒規(guī)格: 96T/48T ,產(chǎn)品別名: 人白介素2受體ELISA檢測(cè)試劑盒,IL-2R 檢測(cè)試劑盒, IL-2R 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。人白介素2受體酶免試劑盒,(IL-2R)ELISA檢測(cè)試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
人白介素2受體(IL-2R)ELISA試劑盒 Human Interleukin-2 receptor,IL-2R ELISA Kit Y00001 96T/48T
人β干擾素(IFN-β/IFNB)ELISA試劑盒 Human Interferon β,IFN-β/IFNB ELISA Kit Y00002 96T/48T
人轉(zhuǎn)化生長(zhǎng)因子β2(TGFβ2)ELISA試劑盒 Human transforming growth factors β2,TGFβ2 ELISA Kit Y00003 96T/48T
人白三烯D4(LTD4)ELISA試劑盒 Human leukotriene D4,LT-D4 ELISA Kit Y00004 96T/48T
人生長(zhǎng)激素釋放因子(GH-RF)ELISA試劑盒 Human growth hormone relasing factor,GH-RF ELISA Kit Y00005 96T/48T
人巨噬細(xì)胞趨化因子(MCF)ELISA試劑盒 Human macrophage chemotatic factor,MCF ELISA Kit Y00006 96T/48T
人α/β干擾素受體(IFN-α/βR)ELISA試劑盒 Human Interferon α/βReceptor,IFN-α/βR ELISA Kit Y00007 96T/48T
人B細(xì)胞生長(zhǎng)因子(BCGF)ELISA試劑盒 Human B cell growth protein,BCGF ELISA Kit Y00008 96T/48T
人B細(xì)胞分化因子(BCDF)ELISA試劑盒 Human B cell differetiation factor,BCDF ELISA Kit Y00009 96T/48T
人可溶性粘附分子(Sam)ELISA試劑盒 Human soluble adhesion molecules,Sam ELISA Kit Y00010 96T/48T
人穿孔素/成孔蛋白(PF/PFP)ELISA試劑盒 Human Perforin/Pore-forming protein,PF/PFP ELISA Kit Y00011 96T/48T
人多效生長(zhǎng)因子(PTN)ELISA試劑盒 Human pleiotrophin,PTN ELISA Kit Y00012 96T/48T
人淋巴細(xì)胞因子ELISA試劑盒 Human lymphocyte factor ELISA Kit Y00013 96T/48T
人神經(jīng)保護(hù)因子(CVNPF)ELISA試劑盒 Human Cobra venom neuronal protective factor,CVNPF ELISA Kit Y00014 96T/48T
人可溶性腫瘤壞死因子α受體(sTNFαR)ELISA試劑盒 Human soluble Tumor Necrosis Factorαreceptor,sTNFαR ELISA Kit Y00015 96T/48T
人可溶性細(xì)胞因子受體(sCKR)ELISA試劑盒 Human soluble cytokine receptor,sCKR ELISA Kit Y00016 96T/48T
人集落刺激因子(CSF)ELISA試劑盒 Human colony-stimulating factor,CSF ELISA Kit Y00017 96T/48T
人凋亡誘導(dǎo)因子(AIF)ELISA試劑盒 Human apoptosis inducing factor,AIF ELISA Kit Y00018 96T/48T
人P鈣黏蛋白/胎盤鈣黏蛋白(P-cad)ELISA試劑盒 Human Placenta Cadherin,P-cad ELISA Kit Y00019 96T/48T
人角化細(xì)胞生長(zhǎng)因子(KGF)ELISA試劑盒 Human Keratinocyte Growth Factor,KGF ELISA Kit Y00020 96T/48T
人血小板衍生生長(zhǎng)因子BB(PDGF-BB)ELISA試劑盒 Human Plaet-Derived Growth Factor-BB,PDGF-BB ELISA Kit Y00021 96T/48T
人淋巴細(xì)胞趨化因子(Lptn/LTN/XCL1)ELISA試劑盒 Human lymphotactin,Lptn/LTN ELISA Kit Y00022 96T/48T
人α干擾素(IFN-α)ELISA試劑盒 Human Interferon α,IFN-α ELISA Kit Y00023 96T/48T
人白介素27(IL-27)ELISA試劑盒 human Interleukin 27,IL-27 ELISA Kit Y00024 96T/48T
人白介素23(IL-23)ELISA試劑盒 Human Interleukin 23,IL-23 ELISA Kit Y00025 96T/48T
人白介素1(IL-1)ELISA試劑盒 Human Interleukin 1,IL-1 ELISA Kit Y00026 96T/48T
人白介素17(IL-17)ELISA試劑盒 Human Interleukin 17,IL-17 ELISA Kit Y00027 96T/48T
人白介素1β (IL-1β)ELISA試劑盒 Human Interleukin 1β,IL-1β ELISA Kit Y00028 96T/48T
人表皮生長(zhǎng)因子(EGF)ELISA試劑盒 Human Epidermal growth factor,EGF ELISA Kit Y00029 96T/48T
人堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)ELISA試劑盒 Human basic fibroblast growth factor,bFGF ELISA Kit Y00030 96T/48T
人可溶性E選擇素(sE-selectin)ELISA試劑盒 Human soluble E-selectin,sE-selectin ELISA Kit Y00031 96T/48T
人可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA試劑盒 Human Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit Y00032 96T/48T
人細(xì)胞間粘附分子2(ICAM-2/CD102)ELISA試劑盒 Human intercellular adhesion molecule 2,ICAM-2 ELISA Kit Y00033 96T/48T
人細(xì)胞間粘附分子3(ICAM-3/CD50)ELISA試劑盒 Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit Y00034 96T/48T
人白介素18(IL-18)ELISA試劑盒 Human Interleukin 18,IL-18 ELISA Kit Y00035 96T/48T
人粘膜相關(guān)上皮趨化因子(MEC/CCL28)ELISA試劑盒 Human mucosae associated epithelia chemokine,MEC ELISA Kit Y00036 96T/48T
人血管內(nèi)皮細(xì)胞生長(zhǎng)因子B(VEGF-B)ELISA試劑盒 Human Vascular Endothelial cell Growth Factor B,VEGF-B ELISA Kit Y00037 96T/48T
人血管內(nèi)皮細(xì)胞生長(zhǎng)因子(VEGF)ELISA試劑盒 Human Vascular Endothelial cell Growth Factor,VEGF ELISA Kit Y00038 96T/48T
人血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒 Human Vascuolar cell adhesion molecule 1,VCAM-1 ELISA Kit Y00039 96T/48T
人腫瘤壞死因子β(TNF-β)ELISA試劑盒 Human Tumor necrosis factor β,TNF-β ELISA Kit Y00040 96T/48T
人腫瘤壞死因子α(TNF-α)ELISA試劑盒 Human Tumor necrosis factor α,TNF-α ELISA Kit Y00041 96T/48T
人轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)ELISA試劑盒 Human Transforming Growth factor β1,TGF-β1 ELISA Kit Y00042 96T/48T
人轉(zhuǎn)化生長(zhǎng)因子α(TGF-α)ELISA試劑盒 Human transforming growth factor α,TGF-α ELISA Kit Y00043 96T/48T
人干細(xì)胞因子/肥大細(xì)胞生長(zhǎng)因子(SCF/MGF)ELISA試劑盒 Human Stem cell factor/mast cell growth factor,SCF/MGF ELISA Kit Y00044 96T/48T
人可溶性CD40配體(sCD40L)ELISA試劑盒 Human Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit Y00045 96T/48T
人可溶性CD30配體(sCD30L)ELISA試劑盒 Human Soluble Cluster of differentiation 30 ligand,sCD30L ELISA Kit Y00046 96T/48T
人P選擇素(P-Selectin/CD62P/GMP140)ELISA試劑盒 Human P-Selectin/CD62P/GMP140 ELISA Kit Y00047 96T/48T
人血血小板衍生生長(zhǎng)因子AB(PDGF-AB)ELISA試劑盒 Human Plaet-Derived Growth Factor AB,PDGF-AB ELISA Kit Y00048 96T/48T
人血血小板衍生生長(zhǎng)因子可溶性受體α(PDGFsR-α)ELISA試劑盒 Human Plaet-Derived Growth Factor Soluble Receptor α,PDGFsR-α ELISA Kit Y00049 96T/48T
人巨噬細(xì)胞來(lái)源的趨化因子(MDC/CCL22)ELISA試劑盒 Human Macrophage-Derived Chemokine,MDC ELISA Kit Y00050 96T/48T
人巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒 Human Macrophage Colony-Stimulating Factor,M-CSF ELISA Kit Y00051 96T/48T
人L選擇素(L-Selectin/CD62L)ELISA試劑盒 Human L-Selectin ELISA Kit Y00052 96T/48T
人白介素9(IL-9)ELISA試劑盒 Human Interleukin 9,IL-9 ELISA Kit Y00053 96T/48T
人白介素8(IL-8/CXCL8)ELISA試劑盒 Human Interleukin 8,IL-8 ELISA Kit Y00054 96T/48T
人白介素6(IL-6)ELISA試劑盒 Human Interleukin 6,IL-6 ELISA Kit Y00055 96T/48T
人白介素-5(IL-5)ELISA試劑盒 Human Interleukin 5,IL-5 ELISA Kit Y00056 96T/48T
人白介素4(IL-4)ELISA試劑盒 Human Interleukin 4,IL-4 ELISA Kit Y00057 96T/48T
人白介素3(IL-3)ELISA試劑盒 Human Interleukin 3,IL-3 ELISA Kit Y00058 96T/48T
人白介素2可溶性受體β鏈(IL-2sRβ )ELISA試劑盒 Human Interleukin 2,IL-2 ELISA Kit Y00059 96T/48T
人白介素2可溶性受體α鏈(IL-2sRα/CD25)ELISA試劑盒 Human soluble interleukin-2 receptor,IL-2sRα ELISA Kit Y00060 96T/48T
人白介素2(IL-2)ELISA試劑盒 Human Interleukin 2,IL-2 ELISA Kit Y00061 96T/48T
人白介素1α(IL-1α )ELISA試劑盒 Human Interleukin 1α,IL-1α ELISA Kit Y00062 96T/48T
人白介素1可溶性受體Ⅱ(IL-1sRⅡ)ELISA試劑盒 Human soluble interleukin-1 receptor Ⅱ,IL-1sRⅡ ELISA Kit Y00063 96T/48T
人白介素1可溶性受體Ⅰ(IL-1sRⅠ)ELISA試劑盒 Human soluble interleukin-1 receptor Ⅰ,IL-1sRⅠ ELISA Kit Y00064 96T/48T
人白介素16(IL-16)ELISA試劑盒 Human Interleukin 16,IL-16 ELISA Kit Y00065 96T/48T
人白介素13(IL-13)ELISA試劑盒 Human Interleukin 13,IL-13 ELISA Kit Y00066 96T/48T
人白介素12(IL-12/P70)ELISA試劑盒 Human Interleukin 12,IL-12/P70 ELISA Kit Y00067 96T/48T
各種類型ELISA測(cè)定時(shí),一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。