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人白介素1α酶免試劑盒,(IL-1α )ELISA檢測試劑盒,英文名:IL-1α ELISA Kit,英文縮寫:IL-1α ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人白介素1αELISA檢測試劑盒,IL-1α 檢測試劑盒,IL-1α 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。人白介素1α酶免試劑盒,(IL-1α )ELISA檢測試劑盒 操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競爭法及定量檢測影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
雞巨噬細(xì)胞替代激活相關(guān)化學(xué)因子1(AmAC-1)ELISA試劑盒Chicken Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISA KitY0305196T/48T
雞免疫核糖核酸(Irna)ELISA試劑盒 Chicken Immune RNA,Irna ELISA KitY0305296T/48T
雞β內(nèi)酰胺酶(β-lactamase)ELISA試劑盒 Chicken β-lactamase ELISA KitY0305396T/48T
雞流行性乙型腦炎抗體IgG(JE IgG)ELISA試劑盒Chicken Japanese Encephalitis IgG,JE IgG ELISA Kit Y0305496T/48T
雞α葡萄糖苷酶(a-Glu)ELISA試劑盒Chicken α-Glucosidase,a-Glu ELISA Kit Y0305596T/48T
雞血小板因子4(PF-4/CXCL4)ELISA試劑盒Chicken Plaet Factor 4,PF-4 ELISA Kit Y0305696T/48T
雞腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)ELISA試劑盒Chicken tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit Y0305796T/48T
雞L苯丙氨酸解氨酶(PAL)ELISA試劑盒 Chicken L-Phenylalanine ammonla-lyase,PAL ELISA Kit Y0305896T/48T
雞煙酰胺腺嘌呤二核苷酸磷酸(NADPH)ELISA試劑盒 Chicken nicotinamide adenine dinucleotide phosphate,NADPH ELISA Kit Y0305996T/48T
雞B因子(BF)ELISA試劑盒Chicken factor B,BF ELISA Kit Y0306096T/48T
雞5核苷酸酶(5-NT)ELISA試劑盒 Chicken 5-Nucleotidase,5-NT ELISA Kit Y0306196T/48T
雞層連蛋白/板層素(LN)ELISA試劑盒 Chicken Laminin,LN ELISA Kit Y0306296T/48T
雞17-酮類固醇(17-KS)ELISA試劑盒Chicken 17-ketosteroids,17-KS ELISA Kit Y0306396T/48T
雞N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA試劑盒Chicken N-acetyl-β-D-glucosaminidase,NAG ELISA Kit Y0306496T/48T
雞神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)ELISA試劑盒Chicken glial fibrillary acidic protein,GFAP ELISA Kit Y0306596T/48T
雞白介素17(IL-17)ELISA試劑盒 Chicken Interleukin 17,IL-17 ELISA Kit Y0306696T/48T
雞1,3-βD葡葡糖苷酶(1,3-βD-Glu)ELISA試劑盒 Chicken 1,3-βD glucosidase,1,3-βD-Glu ELISA Kit Y0306796T/48T
雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒Chicken soluble endothelial protein C receptor,sEPCR ELISA KitY0306896T/48T
兔雙氫睪酮(DHT)ELISA試劑盒 Rabbit Dihydro testosterone,DHT ELISA KitY0306996T/48T
兔核因子κB亞基p65親和肽(NF-κB p65)ELISA試劑盒Rabbit nuclear factor-κB p65,NF-κB p65 ELISA KitY0307096T/48T
兔粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒Rabbit Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit Y0307196T/48T
兔血清淀粉樣蛋白A(SAA)ELISA試劑盒Rabbit Serum amyloid A,SAA ELISA KitY0307296T/48T
兔結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)ELISA試劑盒Rabbit Haptoglobin,Hpt/HP ELISA KitY0307396T/48T
兔子α2抗纖溶酶(α2-AP)ELISA試劑盒 Rabbit α2-Antiplasmin,α2-AP ELISA KitY0307496T/48T
兔子纖溶酶原(Plg)ELISA試劑盒 Rabbit Plasminogen,Plg ELISA KitY0307596T/48T
兔骨形成蛋白(BMPs)ELISA試劑盒 Rabbit bone morphogenetic proteins,BMPs ELISA Kit Y0307696T/48T
兔骨保護(hù)素(OPG)ELISA試劑盒 Rabbit Osteoprotegerin,OPG ELISA Kit Y0307796T/48T
兔胰淀素(Amylin)ELISA試劑盒 Rabbit Amylin ELISA Kit Y0307896T/48T
兔心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)ELISA試劑盒Rabbit cardiac troponin Ⅰ,cTn-ⅠELISA KitY0307996T/48T
兔核因子κB受體活化因子配基(RANKL)ELISA試劑盒 Rabbit receptor activator of nuclear factor kappa B ligand,RANKL ELISA Kit Y0308096T/48T
兔乳鐵傳遞蛋白/乳鐵蛋白(LF/LTF)ELISA試劑盒 Rabbit Lactoferrin,LTF/LF ELISA KitY0308196T/48T
兔心肌特異性肌鈣蛋白T(cTnT)ELISA試劑盒 Rabbit cardiac isoform of Tropnin T,cTnT ELISA KitY0308296T/48T
兔溶菌酶(LZM)ELISA試劑盒 Rabbit Lysozyme,LZM ELISA KitY0308396T/48T
兔子催乳素(PRL)ELISA試劑盒Rabbit prolactin,PRL ELISA KitY0308496T/48T
兔子促黃體激素(LH)ELISA試劑盒 Rabbit luteotropic hormone,LH ELISA KitY0308596T/48T
兔抗腦組織抗體(ABAb)ELISA試劑盒 Rabbit anti-brain tissue antibody,ABAb ELISA Kit Y0308696T/48T
兔p53(p53)ELISA試劑盒 Rabbit p53/tumor protein,p53/TP53 ELISA Kit Y0308796T/48T
兔Bcl-2相關(guān)X蛋白(BAX)ELISA試劑盒 Rabbit Bcl-2 Assaciated X protein,BAX ELISA Kit Y0308896T/48T
兔阿霉素(ADR)ELISA試劑盒Rabbit adramycin,ADR ELISA KitY0308996T/48T
兔基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)ELISA試劑盒Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA KitY0309096T/48T
兔抑瘤素M(OSM)ELISA試劑盒Rabbit Oncostatin-M,OSM ELISA KitY0309196T/48T
兔血管生成素2(ANG-2)ELISA試劑盒Rabbit Angiopoietin 2,ANG-2 ELISA KitY0309296T/48T
兔血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒Rabbit angiotension Ⅱ,ANG-Ⅱ ELISA KitY0309396T/48T
兔可溶性凋亡相關(guān)因子(sFAS/Apo-1)ELISA試劑盒 Rabbit soluble Factor-related Apoptosis,sFAS/Apo-1 ELISA KitY0309496T/48T
兔6酮前列腺素F1a(6-keto-PGF1a)ELISA試劑盒Rabbit 6-keto-prostaglandin F1a,6-K-PGF1a ELISA KitY0309596T/48T
兔血栓素B2(TXB2)ELISA試劑盒Rabbit Thromboxane B2,TX-B2 ELISA KitY0309696T/48T
兔血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)ELISA試劑盒Rabbit Vascuolar cell adhesion molecule 1,VCAM-1 ELISA KitY0309796T/48T
兔血纖蛋白原(Fbg)ELISA試劑盒人白介素1α酶免試劑盒,(IL-1α )ELISA檢測試劑盒 Rabbit Fibrinogen,Fbg ELISA KitY0309896T/48T
兔子D二聚體(D2D)ELISA試劑盒Rabbit D-Dimer,D2D ELISA KitY0309996T/48T
兔子白介素1可溶性受體I (IL-1sR Ⅰ)ELISA試劑盒Rabbit soluble interleukin-1 receptor Ⅰ,IL-1sR Ⅰ ELISA KitY0310096T/48T
各種類型ELISA測定時(shí),人白介素1α酶免試劑盒,(IL-1α )ELISA檢測試劑盒 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。