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小鼠骨退化特異標(biāo)志物酶免試劑盒,(CTX-2)ELISA檢測試劑盒,英文名:CTX-2 ELISA Kit,英文縮寫:CTX-2 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠骨退化特異標(biāo)志物ELISA檢測試劑盒,CTX-2 檢測試劑盒,CTX-2 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠骨退化特異標(biāo)志物酶免試劑盒,(CTX-2)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
雙腎上腺皮質(zhì)激素(DCX)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Doublecortin (DCX)48T/96TY13425Mus musculus (Mouse)
組氨酸豐富糖蛋白(HRG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Histidine Rich Glycoprotein (HRG)48T/96TY13426Homo sapiens (Human)
含MOCO硫化酶羧基端域蛋白1(MOSC1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for MOCO Sulphurase C-Terminal Domain Containing Protein 1 (MOSC1)48T/96TY13427Homo sapiens (Human)
含MANSC域蛋白1(MANSC1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for MANSC Domain Containing Protein 1 (MANSC1)48T/96TY13428Homo sapiens (Human)
利鈉肽受體2(NPR2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Natriuretic Peptide Receptor 2 (NPR2)48T/96TY13429Homo sapiens (Human)
碳酸酐酶Ⅳ(CA4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Carbonic Anhydrase IV (CA4)48T/96TY13430Homo sapiens (Human)
對氧磷酶3(PON3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Paraoxonase 3 (PON3)48T/96TY13431Homo sapiens (Human)
血管非炎性蛋白3(VNN3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vanin 3 (VNN3)48T/96TY13432Homo sapiens (Human)
脂素1(LPIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lipin 1 (LPIN1)48T/96TY13433Rattus norvegicus (Rat)
羧肽酶B1(CPB1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Carboxypeptidase B1, Tissue (CPB1)48T/96TY13434Rattus norvegicus (Rat)
組蛋白脫乙酰基酶6(HDAC6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Histone Deacetylase 6 (HDAC6)48T/96TY13435Homo sapiens (Human)
Bcl2關(guān)聯(lián)永生基因3(BAG3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Bcl2 Associated Athanogene 3 (BAG3)48T/96TY13436Homo sapiens (Human)
胰島素降解酶(IDE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Degrading Enzyme (IDE)48T/96TY13437Rattus norvegicus (Rat)
ⅩⅤ型膠原(COL15)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Collagen Type XV (COL15)48T/96TY13438Homo sapiens (Human)
絲織蛋白1(DBN1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Drebrin 1 (DBN1)48T/96TY13439Homo sapiens (Human)
墨蝶呤還原酶(SPR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Sepiapterin Reductase (SPR)48T/96TY13440Homo sapiens (Human)
序列相似家族135成員B(FAM135B)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Family With Sequence Similarity 135, Member B (FAM135B)48T/96TY13441Mus musculus (Mouse)
甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)48T/96TY13442Mus musculus (Mouse)
分泌粒蛋白Ⅴ(SCG5)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Secretogranin V (SCG5)48T/96TY13443Homo sapiens (Human)
煙堿型膽堿受體α5(CHRNα5)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5)48T/96TY13444Homo sapiens (Human)
生長分化因子2(GDF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Growth Differentiation Factor 2 (GDF2)48T/96TY13445Mus musculus (Mouse)
含卷曲螺旋C2域蛋白1A(CC2D1A)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Coiled Coil And C2 Domain Containing Protein 1A (CC2D1A)48T/96TY13446Homo sapiens (Human)
鋅結(jié)合α2-糖蛋白1(αZGP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1)48T/96TY13447Mus musculus (Mouse)
斯鈣素1(STC1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Stanniocalcin 1 (STC1)48T/96TY13448Rattus norvegicus (Rat)
絲蛋白結(jié)合LIM蛋白1(FBLIM1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Filamin Binding LIM Protein 1 (FBLIM1)48T/96TY13449Rattus norvegicus (Rat)
L-型電壓依賴性鈣離子通道α1D亞基(CACNα1D)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D)48T/96TY13450Homo sapiens (Human)
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY13451Bos taurus; Bovine (Cattle)
神經(jīng)纖維素2(NF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neurofibromin 2 (NF2)48T/96TY13452Homo sapiens (Human)
母系蛋白2(MATN2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Matrilin 2 (MATN2)48T/96TY13453Mus musculus (Mouse)
皮質(zhì)酮(CORT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Corticosterone (CORT)48T/96TY13454General
抗利尿激素(ADH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Antidiuretic Hormone (ADH)48T/96TY13455Mus musculus (Mouse)
酪胺酸酶(TYR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tyrosinase (TYR)48T/96TY13456Rattus norvegicus (Rat)
組織蛋白酶G(CTSG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cathepsin G (CTSG)48T/96TY13457Homo sapiens (Human)
肝細(xì)胞生長因子受體(HGFR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hepatocyte Growth Factor Receptor (HGFR)48T/96TY13458Homo sapiens (Human)
二氫乳清酸脫氫酶(DHODH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Dihydroorotate Dehydrogenase (DHODH)48T/96TY13459Homo sapiens (Human)
精氨酸加壓素原(VP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Preprovasopressin (VP)48T/96TY13460Mus musculus (Mouse)
白介素5受體α(IL5Rα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 5 Receptor Alpha (IL5Ra)48T/96TY13461Mus musculus (Mouse)
應(yīng)激誘導(dǎo)磷蛋白1(STIP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Stress Induced Phosphoprotein 1 (STIP1)48T/96TY13462Homo sapiens (Human)
硬脂酰輔酶A去飽和酶(SCD)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Stearoyl Coenzyme A Desaturase (SCD)48T/96TY13463Homo sapiens (Human)
成纖維細(xì)胞生長因子21(FGF21)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 21 (FGF21)48T/96TY13464Sus scrofa; Porcine (Pig)
補(bǔ)體成分4d(C4d)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Complement Component 4d (C4d)48T/96TY13465Homo sapiens (Human)
激肽釋放酶6(KLK6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Kallikrein 6 (KLK6)48T/96TY13466Rattus norvegicus (Rat)
嗜鉻蛋白B(CHGB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Chromogranin B (CHGB)48T/96TY13467Rattus norvegicus (Rat)
胰島素樣生長因子酸不穩(wěn)定亞基(IGFALS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS)48T/96TY13468Homo sapiens (Human)
血管緊張素Ⅱ(ANGⅡ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Angiotensin II (AngII)48T/96TY13469Bos taurus; Bovine (Cattle)
雙特異性磷酸酶6(DUSP6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Dual Specificity Phosphatase 6 (DUSP6)48T/96TY13470Homo sapiens (Human)
Nodal同源物(NODAL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nodal Homolog (NODAL)48T/96TY13471Mus musculus (Mouse)
Nei內(nèi)切核酸酶Ⅷ樣蛋白1(NEIL1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nei Endonuclease VIII Like Protein 1 (NEIL1)48T/96TY13472Homo sapiens (Human)
黑素瘤細(xì)胞黏附分子(MCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Melanoma Cell Adhesion Molecule (MCAM)48T/96TY13473Mus musculus (Mouse)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY13474Homo sapiens (Human)
Dicer酶1(DICER1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Dicer 1, Ribonuclease Type III (DICER1)48T/96TY13475Homo sapiens (Human)
腦多巴胺神經(jīng)營養(yǎng)因子(CDNF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cerebral Dopamine Neurotrophic Factor (CDNF)48T/96TY13476Homo sapiens (Human)
神經(jīng)肽FF(NPFF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neuropeptide FF (NPFF)48T/96TY13477Homo sapiens (Human)
血管緊張素轉(zhuǎn)化酶2(ACE2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)48T/96TY13478Mus musculus (Mouse)
孕烷X受體(PXR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Pregnane X Receptor (PXR)48T/96TY13479Rattus norvegicus (Rat)
環(huán)氧化合物水解酶4(EPHX4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epoxide Hydrolase 4 (EPHX4)48T/96T Y13480Mus musculus (Mouse)
肌肉磷酸果糖激酶(PFKM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phosphofructokinase, Muscle (PFKM)48T/96TY13481Homo sapiens (Human)
肌肉磷酸果糖激酶(PFKM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phosphofructokinase, Muscle (PFKM)48T/96TY13482Rattus norvegicus (Rat)
各種類型ELISA測定時(shí), 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。