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小鼠腦源性神經(jīng)營養(yǎng)因子酶免試劑盒,(BDNF)ELISA檢測試劑盒,英文名:BDNF ELISA Kit,英文縮寫:BDNF ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠腦源性神經(jīng)營養(yǎng)因子ELISA檢測試劑盒,BDNF 檢測試劑盒,BDNF 檢測試劑盒
ELISA實(shí)驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠腦源性神經(jīng)營養(yǎng)因子酶免試劑盒,(BDNF)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
E1A激活基因阻遏子1(CREG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cellular Repressor Of E1A Stimulated Genes 1 (CREG1)48T/96TY13735Mus musculus (Mouse)
維甲酸受體α(RARα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinoic Acid Receptor Alpha (RARa)48T/96TY13736Rattus norvegicus (Rat)
白介素33(IL33)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 33 (IL33)48T/96TY13737Rattus norvegicus (Rat)
白細(xì)胞衍生趨化因子1(LECT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukocyte Cell Derived Chemotaxin 1 (LECT1)48T/96TY13738Homo sapiens (Human)
輕肽鐵蛋白(FTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ferritin, Light Polypeptide (FTL)48T/96TY13739Rattus norvegicus (Rat)
沉默調(diào)節(jié)蛋白6(SIRT6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sirtuin 6 (SIRT6)48T/96TY13740Homo sapiens (Human)
連環(huán)蛋白β1(CTNNβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Catenin Beta 1 (CTNNb1)48T/96TY13741Rattus norvegicus (Rat)
鈣蛋白酶1(CAPN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calpain 1, Large Subunit (CAPN1)48T/96TY13742Homo sapiens (Human)
糖原合酶激酶3β(GSK3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycogen Synthase Kinase 3 Beta (GSK3b)48T/96TY13743Rattus norvegicus (Rat)
線粒體外膜轉(zhuǎn)位酶70A(TOMM70A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A)48T/96TY13744Homo sapiens (Human)
轉(zhuǎn)甲狀腺素蛋白(TTR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transthyretin (TTR)48T/96TY13745Sus scrofa; Porcine (Pig)
分揀連接蛋白13(SNX13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sorting Nexin 13 (SNX13)48T/96TY13746Homo sapiens (Human)
分揀連接蛋白17(SNX17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sorting Nexin 17 (SNX17)48T/96TY13747Homo sapiens (Human)
微纖絲關(guān)聯(lián)蛋白4(MFAP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Microfibrillar Associated Protein 4 (MFAP4)48T/96TY13748Homo sapiens (Human)
腦多巴胺神經(jīng)營養(yǎng)因子(CDNF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cerebral Dopamine Neurotrophic Factor (CDNF)48T/96TY13749Rattus norvegicus (Rat)
髓細(xì)胞觸發(fā)受體2(TREM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)48T/96TY13750Mus musculus (Mouse)
細(xì)胞程序性死亡蛋白6(PDCD6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Programmed Cell Death Protein 6 (PDCD6)48T/96TY13751Homo sapiens (Human)
細(xì)胞程序性死亡蛋白6相互作用蛋白(PDCD6IP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP)48T/96TY13752Homo sapiens (Human)
纖調(diào)蛋白(FMOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibromodulin (FMOD)48T/96TY13753Rattus norvegicus (Rat)
Ⅴ型膠原α2(COL5α2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type V Alpha 2 (COL5a2)48T/96TY13754Mus musculus (Mouse)
細(xì)胞色素P450家族成員1A2(CYP1A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 1A2 (CYP1A2)48T/96TY13755Homo sapiens (Human)
C1q腫瘤壞死因子相關(guān)蛋白9(C1QTNF9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9)48T/96TY13756Homo sapiens (Human)
角蛋白16(KRT16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 16 (KRT16)48T/96TY13757Homo sapiens (Human)
層粘連蛋白γ1(LAMγ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Laminin Gamma 1 (LAMC1)48T/96TY13758Homo sapiens (Human)
Smad同源物2(Smad2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 2 (Smad2)48T/96TY13759Mus musculus (Mouse)
神經(jīng)外營養(yǎng)蛋白1(NXPH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurexophilin 1 (NXPH1)48T/96TY13760Homo sapiens (Human)
唾液酸酶2(SIAL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialidase 2, Cytosolic (SIAL2)48T/96TY13761Rattus norvegicus (Rat)
肽基精氨酸脫亞氨酶Ⅵ(PADI6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peptidyl Arginine Deiminase Type VI (PADI6)48T/96TY13762Homo sapiens (Human)
原肌球調(diào)節(jié)蛋白3(TMOD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tropomodulin 3 (TMOD3)48T/96TY13763Mus musculus (Mouse)
彈性蛋白微原纖維界面因子2(EMILIN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastin Microfibril Interface Located Protein 2 (EMILIN2)48T/96TY13764Mus musculus (Mouse)
白介素12受體β2(IL2Rβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 12 Receptor Beta 2 (IL12Rb2)48T/96TY13765Homo sapiens (Human)
組胺H3受體(HRH3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histamine Receptor H3 (HRH3)48T/96TY13766Rattus norvegicus (Rat)
角蛋白81(KRT81)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 81 (KRT81)48T/96TY13767Homo sapiens (Human)
分泌球蛋白家族2A成員2(SCGB2A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretoglobin Family 2A, Member 2 (SCGB2A2)48T/96TY13768Homo sapiens (Human)
肌球蛋白輕鏈9(MYL9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Light Chain 9, Regulatory (MYL9)48T/96TY13769Homo sapiens (Human)
半胱氨酸豐富跨膜BMP調(diào)節(jié)因子1(CRIM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1)48T/96TY13770Homo sapiens (Human)
幾丁質(zhì)酶3樣蛋白2(CHI3L2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chitinase 3 Like Protein 2 (CHI3L2)48T/96TY13771Homo sapiens (Human)
視黃醇結(jié)合蛋白3(RBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinol Binding Protein 3, Interstitial (RBP3)48T/96TY13772Homo sapiens (Human)
CD226分子(CD226)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 226 (CD226)48T/96TY13773Homo sapiens (Human)
蘭尼定受體1(RYR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1)48T/96TY13774Rattus norvegicus (Rat)
甲狀腺激素反應(yīng)基因(THRSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thyroid Hormone Responsive (THRSP)48T/96TY13775Homo sapiens (Human)
癌胚抗原相關(guān)細(xì)胞粘附分子8(CEACAM8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8)48T/96TY13776Homo sapiens (Human)
肌球蛋白重鏈8(MYH8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8)48T/96TY13777Rattus norvegicus (Rat)
尼克酰胺-N-甲基轉(zhuǎn)移酶(NNMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nicotinamide-N-Methyltransferase (NNMT)48T/96TY13778Mus musculus (Mouse)
神經(jīng)源素3(NEUROG3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurogenin 3 (NEUROG3)48T/96TY13779Mus musculus (Mouse)
無翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)48T/96TY13780Rattus norvegicus (Rat)
10kDa干擾素γ誘導(dǎo)蛋白(IP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10)48T/96TY13781Bos taurus; Bovine (Cattle)
多聚免疫球蛋白受體(PIGR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Polymeric Immunoglobulin Receptor (PIGR)48T/96TY13782Bos taurus; Bovine (Cattle)
S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)48T/96TY13783Bos taurus; Bovine (Cattle)
S100鈣結(jié)合蛋白A9(S100A9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)48T/96TY13784Bos taurus; Bovine (Cattle)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。