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小鼠破骨細(xì)胞分化因子酶免試劑盒,(ODF)ELISA檢測試劑盒 ,英文名:ODF ELISA Kit ,英文縮寫:ODF ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠破骨細(xì)胞分化因子ELISA檢測試劑盒,ODF 檢測試劑盒, ODF 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠破骨細(xì)胞分化因子酶免試劑盒,(ODF)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
肌肽合酶1(CARNS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnosine Synthase 1 (CARNS1)48T/96TY16385Mus musculus (Mouse)
抗髓過氧化物酶抗體(Anti-MPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Myeloperoxidase Antibody (Anti-MPO)48T/96TY16386Homo sapiens (Human)
纖維蛋白肽A(FPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinopeptide A (FPA)48T/96TY16387Rattus norvegicus (Rat)
黏膜地址素細(xì)胞黏附分子(MAdCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1)48T/96TY16388Mus musculus (Mouse)
D-氨基酸氧化酶(DAO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for D-Amino Acid Oxidase (DAO)48T/96TY16389Homo sapiens (Human)
MutL同源物1(MLH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for MutL Homolog 1 (MLH1)48T/96TY16390Homo sapiens (Human)
磷酸烯醇式丙酮酸羧激酶1(PCK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)48T/96TY16391Rattus norvegicus (Rat)
Kruppel樣因子2(KLF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kruppel Like Factor 2, Lung (KLF2)48T/96TY16392Rattus norvegicus (Rat)
含卵巢癌免疫反應(yīng)抗原域蛋白2(OCIAD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ovarian Cancer Immuno Reactive Antigen Domain Containing Protein 2 (OCIAD2)48T/96TY16393Homo sapiens (Human)
p21蛋白激活激酶4(PAK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for p21 Protein Activated Kinase 4 (PAK4)48T/96TY16394Homo sapiens (Human)
70kDa熱休克蛋白5(HSPA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)48T/96TY16395Rattus norvegicus (Rat)
神經(jīng)激肽A(NKA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurokinin A (NKA)48T/96TY16396Homo sapiens (Human)
低密度脂蛋白受體銜接蛋白1(LDLRAP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1)48T/96TY16397Mus musculus (Mouse)
糖皮質(zhì)激素受體β(GRβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucocorticoid Receptor Beta (GRb)48T/96TY16398Mus musculus (Mouse)
糖皮質(zhì)激素受體α(GRα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucocorticoid Receptor Alpha (GRa)48T/96TY16399Mus musculus (Mouse)
再生胰島衍生蛋白3α(REG3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 3 Alpha (REG3a)48T/96TY16400Mus musculus (Mouse)
前列腺凋亡應(yīng)答因子4(PAR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostate Apoptosis Response 4 (PAR4)48T/96TY16401Homo sapiens (Human)
G蛋白偶聯(lián)受體120(GPR120)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for G Protein Coupled Receptor 120 (GPR120)48T/96TY16402Mus musculus (Mouse)
膽囊收縮素(CCK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholecystokinin (CCK)48T/96TY16403Canis familiaris; Canine (Dog)
激活素A受體ⅡA(ACVR2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activin A Receptor Type II A (ACVR2A)48T/96TY16404Homo sapiens (Human)
膽綠素還原酶A(BLVRA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Biliverdin Reductase A (BLVRA)48T/96TY16405Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子受體1(FGFR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor Receptor 1 (FGFR1)48T/96TY16406Rattus norvegicus (Rat)
黑色素瘤優(yōu)先表達(dá)抗原(PRAME)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Preferentially Expressed Antigen In Melanoma (PRAME)48T/96TY16407Homo sapiens (Human)
多聚ADP核糖聚合酶(PARP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Poly ADP Ribose Polymerase (PARP)48T/96TY16408Homo sapiens (Human)
FK506結(jié)合蛋白12雷帕霉素關(guān)聯(lián)蛋白(FRAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP)48T/96TY16409Homo sapiens (Human)
E1A結(jié)合蛋白P300(EP300)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for E1A Binding Protein P300 (EP300)48T/96TY16410Mus musculus (Mouse)
E1A結(jié)合蛋白P300(EP300)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for E1A Binding Protein P300 (EP300)48T/96TY16411Rattus norvegicus (Rat)
蛋白激酶Bα(PKBα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase B Alpha (PKBa)48T/96TY16412Homo sapiens (Human)
氨乙酰丙酸δ脫水酶(ALAD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aminolevulinate Delta Dehydratase (ALAD)48T/96TY16413Rattus norvegicus (Rat)
F-框蛋白32(FBXO32)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for F-Box Protein 32 (FBXO32)48T/96TY16414Homo sapiens (Human)
多胺氧化酶(PAOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Polyamine Oxidase (PAOX)48T/96TY16415Mus musculus (Mouse)
β-1,4-N-乙酰半乳糖胺基轉(zhuǎn)移酶2(β4GALNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2)48T/96TY16416Homo sapiens (Human)
V-Erb B2紅白血病病毒癌基因同源物3(ErbB3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3)48T/96TY16417Mus musculus (Mouse)
CCAAT增強子結(jié)合蛋白β(CEBPβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CCAAT/Enhancer Binding Protein Beta (CEBPb)48T/96TY16418Mus musculus (Mouse)
CD109分子(CD109)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 109 (CD109)48T/96TY16419Homo sapiens (Human)
Twist轉(zhuǎn)錄因子(TWIST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Twist Transcription Factor (TWIST)48T/96TY16420Homo sapiens (Human)
FK506結(jié)合蛋白1A(FKBP1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for FK506 Binding Protein 1A (FKBP1A)48T/96TY16421Homo sapiens (Human)
興奮性氨基酸轉(zhuǎn)運蛋白2(EAAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Excitatory Amino Acid Transporter 2 (EAAT2)48T/96TY16422Homo sapiens (Human)
蛋白二硫化物異構(gòu)酶A4(PDIA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Disulfide Isomerase A4 (PDIA4)48T/96TY16423Homo sapiens (Human)
AN1-型鋅指域蛋白6(ZFAND6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6)48T/96TY16424Homo sapiens (Human)
類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tryptase (TPS)48T/96TY16425Equus caballus; Equine (Horse)
煙堿型膽堿受體β3(CHRNβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3)48T/96TY16426Homo sapiens (Human)
靜止素Q6硫基氧化酶1(QSOX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)48T/96TY16427Mus musculus (Mouse)
成纖維細(xì)胞生長因子受體3(FGFR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor Receptor 3 (FGFR3)48T/96TY16428Mus musculus (Mouse)
腺苷A2a受體(ADORA2a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenosine A2a Receptor (ADORA2a)48T/96TY16429Homo sapiens (Human)
甘露糖磷酸異構(gòu)酶(MPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Phosphate Isomerase (MPI)48T/96TY16430Homo sapiens (Human)
趨化因子C-C-基元配體1(CCL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Ligand 1 (CCL1)48T/96TY16431Mus musculus (Mouse)
細(xì)胞外基質(zhì)磷酸糖蛋白(MEPE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Matrix Extracellular Phosphoglycoprotein (MEPE)48T/96TY16432Rattus norvegicus (Rat)
淋巴細(xì)胞功能關(guān)聯(lián)抗原1α(LFA1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lymphocyte Function Associated Antigen 1 Alpha (LFA1a)48T/96TY16433Rattus norvegicus (Rat)
含纈酪肽蛋白(VCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Valosin Containing Protein (VCP)48T/96TY16434Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。