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小鼠生長激素釋放肽ghrelin酶免試劑盒,(GHRP-Ghrelin)ELISA檢測試劑盒 ,英文名:GHRP-Ghrelin ELISA Kit ,英文縮寫:GHRP-Ghrelin ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠生長激素釋放肽ghrelinELISA檢測試劑盒,GHRP-Ghrelin 檢測試劑盒, GHRP-Ghrelin 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠生長激素釋放肽ghrelin酶免試劑盒,(GHRP-Ghrelin)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
心型脂肪酸結(jié)合蛋白(FABP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3)48T/96TY16535Mus musculus (Mouse)
胞外5'-核苷酸酶(NT5E)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 5'-Nucleotidase, Ecto (NT5E)48T/96TY16536Rattus norvegicus (Rat)
早老素2(PS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Presenilin 2 (PS2)48T/96TY16537Rattus norvegicus (Rat)
Bcl2關(guān)聯(lián)X蛋白(Bax)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bcl2 Associated X Protein (Bax)48T/96TY16538Bos taurus; Bovine (Cattle)
腺苷酸環(huán)化酶10(ADCY10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 10, Soluble (ADCY10)48T/96TY16539Mus musculus (Mouse)
殺傷細(xì)胞免疫球蛋白樣受體2DS4(KIR2DS4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4)48T/96TY16540Homo sapiens (Human)
白介素22(IL22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 22 (IL22)48T/96TY16541Bos taurus; Bovine (Cattle)
過氧化物酶體增殖物激活受體δ(PPARδ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxisome Proliferator Activated Receptor Delta (PPARd)48T/96TY16542Mus musculus (Mouse)
組蛋白脫乙?;?(HDAC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histone Deacetylase 1 (HDAC1)48T/96TY16543Mus musculus (Mouse)
甲狀旁腺素受體1(PTHR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Parathyroid Hormone Receptor 1 (PTHR1)48T/96TY16544Homo sapiens (Human)
輕肽鐵蛋白(FTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ferritin, Light Polypeptide (FTL)48T/96TY16545Mus musculus (Mouse)
骨骼肌慢肌肌鈣蛋白T(TNNT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1)48T/96TY16546Mus musculus (Mouse)
骨骼肌慢肌肌鈣蛋白T(TNNT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1)48T/96TY16547Rattus norvegicus (Rat)
饑餓素/肥胖抑制素前激素原(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ghrelin/Obestatin Preprohormone (GHRL)48T/96TY16548Rattus norvegicus (Rat)
20S-蛋白酶體(20S-PSM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 20S-Proteasome (20S-PSM)48T/96TY16549Homo sapiens (Human)
20S-蛋白酶體(20S-PSM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 20S-Proteasome (20S-PSM)48T/96TY16550Mus musculus (Mouse)
蛋白酶體亞基α1(PSMα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proteasome Subunit Alpha Type 1 (PSMa1)48T/96TY16551Homo sapiens (Human)
肌球蛋白重鏈4(MYH4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 4, Skeletal Muscle (MYH4)48T/96TY16552Homo sapiens (Human)
肌球蛋白重鏈7(MYH7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)48T/96TY16553Homo sapiens (Human)
肌球蛋白重鏈6(MYH6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)48T/96TY16554Homo sapiens (Human)
5-羥色胺受體2A(HTR2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 5-Hydroxytryptamine Receptor 2A (HTR2A)48T/96TY16555Homo sapiens (Human)
90kDa熱休克蛋白αB1(HSP90αB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Alpha B1 (HSP90aB1)48T/96TY16556Bos taurus; Bovine (Cattle)
腫瘤壞死因子配體超家族成員9(TNFSF9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9)48T/96TY16557Homo sapiens (Human)
核受體相關(guān)蛋白1(NURR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Receptor Related Protein 1 (NURR1)48T/96TY16558Homo sapiens (Human)
腎上腺素能受體β2(ADRβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adrenergic Receptor Beta 2 (ADRb2)48T/96TY16559Rattus norvegicus (Rat)
腎上腺素能受體β3(ADRβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adrenergic Receptor Beta 3 (ADRb3)48T/96TY16560Rattus norvegicus (Rat)
重肽神經(jīng)絲蛋白(NEFH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurofilament, Heavy Polypeptide (NEFH)48T/96TY16561Rattus norvegicus (Rat)
鈉/葡萄糖協(xié)同轉(zhuǎn)運蛋白1(SGLT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sodium/Glucose Cotransporter 1 (SGLT1)48T/96TY16562Mus musculus (Mouse)
鈉/葡萄糖協(xié)同轉(zhuǎn)運蛋白2(SGLT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sodium/Glucose Cotransporter 2 (SGLT2)48T/96TY16563Rattus norvegicus (Rat)
睪丸陰離子轉(zhuǎn)運蛋白1(TAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Testis Anion Transporter 1 (TAT1)48T/96TY16564Homo sapiens (Human)
再生胰島衍生蛋白1β(REG1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 1 Beta (REG1b)48T/96TY16565Mus musculus (Mouse)
AMPA離子能谷氨酸受體1(GRIA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)48T/96TY16566Homo sapiens (Human)
AMPA離子能谷氨酸受體2(GRIA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)48T/96TY16567Homo sapiens (Human)
AMPA離子能谷氨酸受體2(GRIA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)48T/96TY16568Mus musculus (Mouse)
AMPA離子能谷氨酸受體3(GRIA3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3)48T/96TY16569Mus musculus (Mouse)
AMPA離子能谷氨酸受體4(GRIA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4)48T/96TY16570Homo sapiens (Human)
AMPA離子能谷氨酸受體4(GRIA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4)48T/96TY16571Mus musculus (Mouse)
組蛋白脫乙酰基酶4(HDAC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histone Deacetylase 4 (HDAC4)48T/96TY16572Mus musculus (Mouse)
線粒體解偶聯(lián)蛋白1(UCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1)48T/96TY16573Homo sapiens (Human)
易位關(guān)聯(lián)膜蛋白1(TRAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Translocation Associated Membrane Protein 1 (TRAM1)48T/96TY16574Homo sapiens (Human)
分泌粒蛋白Ⅱ(SCG2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretogranin II (SCG2)48T/96TY16575Mus musculus (Mouse)
腺苷A1受體(ADORA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenosine A1 Receptor (ADORA1)48T/96TY16576Rattus norvegicus (Rat)
黑素瘤缺乏因子1(AIM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Absent In Melanoma 1 (AIM1)48T/96TY16577Homo sapiens (Human)
煙酸酰胺腺嘌呤二核苷酸磷酸(NAADP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)48T/96TY16578General
銅離子轉(zhuǎn)運ATP酶β肽(ATP7β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Cu++ Transporting Beta Polypeptide (ATP7b)48T/96TY16579Mus musculus (Mouse)
TNF受體關(guān)聯(lián)因子3(TRAF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TNF Receptor Associated Factor 3 (TRAF3)48T/96TY16580Homo sapiens (Human)
TNF受體關(guān)聯(lián)因子5(TRAF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TNF Receptor Associated Factor 5 (TRAF5)48T/96TY16581Homo sapiens (Human)
Toll樣受體銜接分子1(TICAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor Adaptor Molecule 1 (TICAM1)48T/96TY16582Homo sapiens (Human)
TANK結(jié)合激酶1(TBK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TANK Binding Kinase 1 (TBK1)48T/96TY16583Homo sapiens (Human)
V-Maf肌肉腱膜纖維肉瘤癌基因同源物(MAF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF)48T/96TY16584Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。