服務(wù)熱線
021-54479081
021-54461587
產(chǎn)品展示PRODUCTS
本產(chǎn)品僅用于科研,不用于臨床
小鼠磷酸化蛋白激酶C酶免試劑盒,(P-PKC)ELISA檢測試劑盒 ,英文名:P-PKC ELISA Kit ,英文縮寫:P-PKC ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠磷酸化蛋白激酶CELISA檢測試劑盒,P-PKC 檢測試劑盒, P-PKC 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠磷酸化蛋白激酶C酶免試劑盒,(P-PKC)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)96TY17246Sus scrofa; Porcine (Pig)
轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)96TY17247Oryctolagus cuniculus (Rabbit)
成纖維細(xì)胞生長因子19(FGF19)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 19 (FGF19)96TY17248Homo sapiens (Human)
成纖維細(xì)胞生長因子19(FGF19)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 19 (FGF19)96TY17249Mus musculus (Mouse)
成纖維細(xì)胞生長因子19(FGF19)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 19 (FGF19)96TY17250Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子21(FGF21)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 21 (FGF21)96TY17251Homo sapiens (Human)
成纖維細(xì)胞生長因子21(FGF21)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 21 (FGF21)96TY17252Mus musculus (Mouse)
成纖維細(xì)胞生長因子21(FGF21)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 21 (FGF21)96TY17253Sus scrofa; Porcine (Pig)
成纖維細(xì)胞生長因子21(FGF21)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 21 (FGF21)96TY17254Rattus norvegicus (Rat)
血小板衍生生長因子D(PDGFD)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor D (PDGFD)96TY17255Homo sapiens (Human)
血小板衍生生長因子D(PDGFD)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor D (PDGFD)96TY17256Mus musculus (Mouse)
血小板衍生生長因子D(PDGFD)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor D (PDGFD)96TY17257Rattus norvegicus (Rat)
血小板衍生生長因子C(PDGFC)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor C (PDGFC)96TY17258Homo sapiens (Human)
血小板衍生生長因子C(PDGFC)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor C (PDGFC)96TY17259Mus musculus (Mouse)
血小板衍生生長因子C(PDGFC)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor C (PDGFC)96TY17260Rattus norvegicus (Rat)
血小板衍生生長因子B(PDGFB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)96TY17261Homo sapiens (Human)
血小板衍生生長因子B(PDGFB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)96TY17262Mus musculus (Mouse)
血小板衍生生長因子B(PDGFB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)96TY17263Sus scrofa; Porcine (Pig)
血小板衍生生長因子B(PDGFB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)96TY17264Rattus norvegicus (Rat)
血小板衍生生長因子受體樣蛋白(PDGFRL)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor Receptor Like Protein (PDGFRL)96TY17265Homo sapiens (Human)
血小板衍生生長因子受體樣蛋白(PDGFRL)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor Receptor Like Protein (PDGFRL)96TY17266Mus musculus (Mouse)
血小板衍生生長因子受體樣蛋白(PDGFRL)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Plaet Derived Growth Factor Receptor Like Protein (PDGFRL)96TY17267Rattus norvegicus (Rat)
生長激素2(GH2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Hormone 2 (GH2)96TY17268Homo sapiens (Human)
生長激素2(GH2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Hormone 2 (GH2)96TY17269Mus musculus (Mouse)
生長激素2(GH2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Hormone 2 (GH2)96TY17270Rattus norvegicus (Rat)
白介素1η(IL1η)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Eta (IL1h)96TY17271Homo sapiens (Human)
白介素1η(IL1η)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Eta (IL1h)96TY17272Mus musculus (Mouse)
白介素1η(IL1η)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Eta (IL1h)96TY17273Rattus norvegicus (Rat)
干擾素ε(IFNε)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interferon Epsilon (IFNe)96TY17274Homo sapiens (Human)
干擾素ε(IFNε)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interferon Epsilon (IFNe)96TY17275Mus musculus (Mouse)
干擾素κ(IFNκ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interferon Kappa (IFNk)96TY17276Homo sapiens (Human)
干擾素κ(IFNκ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interferon Kappa (IFNk)96TY17277Mus musculus (Mouse)
干擾素κ(IFNκ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interferon Kappa (IFNk)96TY17278Rattus norvegicus (Rat)
EB病毒誘導(dǎo)蛋白3(EBI3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Epstein Barr Virus Induced Protein 3 (EBI3)96TY17279Homo sapiens (Human)
EB病毒誘導(dǎo)蛋白3(EBI3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Epstein Barr Virus Induced Protein 3 (EBI3)96TY17280Mus musculus (Mouse)
EB病毒誘導(dǎo)蛋白3(EBI3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Epstein Barr Virus Induced Protein 3 (EBI3)96TY17281Rattus norvegicus (Rat)
白介素17C(IL17C)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 17C (IL17C)96TY17282Homo sapiens (Human)
白介素17C(IL17C)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 17C (IL17C)96TY17283Mus musculus (Mouse)
白介素17C(IL17C)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 17C (IL17C)96TY17284Rattus norvegicus (Rat)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for N-Acetyl Beta-D-Glucosaminidase (NAGase)96TY17285Homo sapiens (Human)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for N-Acetyl Beta-D-Glucosaminidase (NAGase)96TY17286Mus musculus (Mouse)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for N-Acetyl Beta-D-Glucosaminidase (NAGase)96TY17287Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 1 (MMP1)96TY17288Bos taurus; Bovine (Cattle)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 1 (MMP1)96TY17289Equus caballus; Equine (Horse)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 1 (MMP1)96TY17290Homo sapiens (Human)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 1 (MMP1)96TY17291Mus musculus (Mouse)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 1 (MMP1)96TY17292Ovis aries; Ovine (Sheep)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 1 (MMP1)96TY17293Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 1 (MMP1)96TY17294Oryctolagus cuniculus (Rabbit)
基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Matrix Metalloproteinase 10 (MMP10)96TY17295Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。