服務(wù)熱線
021-54479081
021-54461587
產(chǎn)品展示PRODUCTS
本產(chǎn)品僅用于科研,不用于臨床
小鼠中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白酶免試劑盒,(NGAL)ELISA檢測試劑盒 ,英文名:NGAL ELISA Kit ,英文縮寫:NGAL ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白ELISA檢測試劑盒,NGAL 檢測試劑盒, NGAL 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白酶免試劑盒,(NGAL)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
白介素33(IL33)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 33 (IL33)96TY17746Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子3(FGF3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 3 (FGF3)96TY17747Homo sapiens (Human)
成纖維細(xì)胞生長因子3(FGF3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 3 (FGF3)96TY17748Mus musculus (Mouse)
成纖維細(xì)胞生長因子3(FGF3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 3 (FGF3)96TY17749Rattus norvegicus (Rat)
顆粒體蛋白(GRN)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Granulin (GRN)96TY17750Homo sapiens (Human)
顆粒體蛋白(GRN)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Granulin (GRN)96TY17751Mus musculus (Mouse)
顆粒體蛋白(GRN)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Granulin (GRN)96TY17752Rattus norvegicus (Rat)
心營養(yǎng)素樣細(xì)胞因子1(CLCF1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)96TY17753Homo sapiens (Human)
心營養(yǎng)素樣細(xì)胞因子1(CLCF1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)96TY17754Mus musculus (Mouse)
心營養(yǎng)素樣細(xì)胞因子1(CLCF1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)96TY17755Rattus norvegicus (Rat)
同種異體移植炎癥因子1(AIF1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Allograft Inflammatory Factor 1 (AIF1)96TY17756Homo sapiens (Human)
同種異體移植炎癥因子1(AIF1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Allograft Inflammatory Factor 1 (AIF1)96TY17757Mus musculus (Mouse)
同種異體移植炎癥因子1(AIF1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Allograft Inflammatory Factor 1 (AIF1)96TY17758Rattus norvegicus (Rat)
左右決定因子1(LEFTY1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Left/Right Determination Factor 1 (LEFTY1)96TY17759Homo sapiens (Human)
左右決定因子1(LEFTY1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Left/Right Determination Factor 1 (LEFTY1)96TY17760Mus musculus (Mouse)
左右決定因子1(LEFTY1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Left/Right Determination Factor 1 (LEFTY1)96TY17761Rattus norvegicus (Rat)
Cerberus蛋白(CER)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cerberus (CER)96TY17762Homo sapiens (Human)
Cerberus蛋白(CER)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cerberus (CER)96TY17763Mus musculus (Mouse)
HtrA絲氨酸肽酶4(HTRA4)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for HtrA Serine Peptidase 4 (HTRA4)96TY17764Homo sapiens (Human)
紅細(xì)胞生成素(EPO)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Erythropoietin (EPO)96TY17765Rattus norvegicus (Rat)
胞外超氧化物歧化酶(SOD3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Superoxide Dismutase 3, Extracellular (SOD3)96TY17766Mus musculus (Mouse)
組蛋白H4(H4)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Histone H4 (H4)96TY17767Mus musculus (Mouse)
半乳糖凝集素9(GAL9)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Galectin 9 (GAL9)96TY17768Homo sapiens (Human)
補(bǔ)體成分5a(C5a)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Component 5a (C5a)96TY17769Rattus norvegicus (Rat)
雌激素受體β(ERβ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Estrogen Receptor Beta (ERb)96TY17770Homo sapiens (Human)
氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP)96TY17771Canis familiaris; Canine (Dog)
Ⅰ型前膠原羧基端原肽(PⅠCP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Procollagen I C-Terminal Propeptide (PICP)96TY17772Homo sapiens (Human)
血小板反應(yīng)蛋白(THBS1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Thrombospondin 1 (THBS1)96TY17773Mus musculus (Mouse)
分泌型免疫球蛋白A(sIgA)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Secretory Immunoglobulin A (sIgA)96TY17774Bos taurus; Bovine (Cattle)
轉(zhuǎn)甲狀腺素蛋白(TTR)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transthyretin (TTR)96TY17775Homo sapiens (Human)
生長分化因子11(GDF11)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Differentiation Factor 11 (GDF11)96TY17776Homo sapiens (Human)
C4結(jié)合蛋白β(C4BPβ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for C4 Binding Protein Beta (C4BPb)96TY17777Homo sapiens (Human)
補(bǔ)體因子P(CFP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Factor P (CFP)96TY17778Homo sapiens (Human)
生長分化因子11(GDF11)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Differentiation Factor 11 (GDF11)96TY17779Mus musculus (Mouse)
核因子κB受體激活因子配基(RANκL)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)96TY17780Rattus norvegicus (Rat)
補(bǔ)體成分1r(C1r)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Component 1, R Subcomponent (C1r)96TY17781Homo sapiens (Human)
生長調(diào)節(jié)致癌基因β(GROβ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Regulated Oncogene Beta (GROb)96TY17782Homo sapiens (Human)
補(bǔ)體成分1s(C1s)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Component 1, S Subcomponent (C1s)96TY17783Homo sapiens (Human)
補(bǔ)體成分8γ(C8γ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Component 8g (C8g)96TY17784Homo sapiens (Human)
博來霉素水解酶(BLMH)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bleomycin Hydrolase (BLMH)96TY17785Homo sapiens (Human)
前梯度蛋白2(AGR2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Anterior Gradient Protein 2 (AGR2)96TY17786Homo sapiens (Human)
絡(luò)絲蛋白(RL)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Reelin (RL)96TY17787Rattus norvegicus (Rat)
甲狀腺激素反應(yīng)基因(THRSP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Thyroid Hormone Responsive (THRSP)96TY17788Homo sapiens (Human)
蛋白C抑制因子(PCI)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Protein C Inhibitor (PCI)96TY17789Homo sapiens (Human)
妊娠相關(guān)血漿蛋白A2(PAPPA2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Pappalysin 2 (PAPPA2)96TY17790Homo sapiens (Human)
補(bǔ)體成分1q子成分C(C1qC)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Component 1, Q Subcomponent C (C1qC)96TY17791Homo sapiens (Human)
泛素羧基端酯酶L1(UCHL1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)96TY17792Rattus norvegicus (Rat)
生長分化因子11(GDF11)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Differentiation Factor 11 (GDF11)96TY17793Rattus norvegicus (Rat)
生長分化因子5(GDF5)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Differentiation Factor 5 (GDF5)96TY17794Homo sapiens (Human)
生長分化因子5(GDF5)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Differentiation Factor 5 (GDF5)96TY17795Mus musculus (Mouse)
各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。